SrasV1, Main, Exploration, bibRecord, 004E54

Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS

Identifieur interne : 004E54 ( Main/Exploration ); précédent : 004E53; suivant : 004E55

Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS

Source :

Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2005.

RBID : Pascal:05-0347652

Descripteurs français

Pascal (Inist)
- Anticorps, Déterminant antigénique, Cartographie, Nucléocapside, Protéine, Syndrome respiratoire aigu sévère, Coronavirus, Immunité humorale, Biochimie, Biologie clinique, Biologie moléculaire.
Wicri :
- topic : Cartographie, Biochimie.

English descriptors

KwdEn :
- Antibody, Antigenic determinant, Biochemistry, Cartography, Clinical biology, Coronavirus, Humoral immunity, Molecular biology, Nucleocapsid, Protein, Severe acute respiratory syndrome.

Abstract

Background: The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease. Methods: We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display. Results: We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1-69, 68-213, 212-341, and 337-422). Conclusion: The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Curation: 000358
to stream PascalFrancis, to step Checkpoint: 000684
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Le document en format XML

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<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
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<author><name sortKey="Yuzhang Wu" sort="Yuzhang Wu" uniqKey="Yuzhang Wu" last="Yuzhang Wu">YUZHANG WU</name>
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</analytic>
<series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
<term>Antigenic determinant</term>
<term>Biochemistry</term>
<term>Cartography</term>
<term>Clinical biology</term>
<term>Coronavirus</term>
<term>Humoral immunity</term>
<term>Molecular biology</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Anticorps</term>
<term>Déterminant antigénique</term>
<term>Cartographie</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Coronavirus</term>
<term>Immunité humorale</term>
<term>Biochimie</term>
<term>Biologie clinique</term>
<term>Biologie moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cartographie</term>
<term>Biochimie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background: The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease. Methods: We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display. Results: We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1-69, 68-213, 212-341, and 337-422). Conclusion: The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Yunfei Liang" sort="Yunfei Liang" uniqKey="Yunfei Liang" last="Yunfei Liang">YUNFEI LIANG</name>
</noRegion>
<name sortKey="Bing Ni" sort="Bing Ni" uniqKey="Bing Ni" last="Bing Ni">BING NI</name>
<name sortKey="Bo Guo" sort="Bo Guo" uniqKey="Bo Guo" last="Bo Guo">BO GUO</name>
<name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
<name sortKey="Jingran Zhou" sort="Jingran Zhou" uniqKey="Jingran Zhou" last="Jingran Zhou">JINGRAN ZHOU</name>
<name sortKey="Liyun Zou" sort="Liyun Zou" uniqKey="Liyun Zou" last="Liyun Zou">LIYUN ZOU</name>
<name sortKey="Qiang Zou" sort="Qiang Zou" uniqKey="Qiang Zou" last="Qiang Zou">QIANG ZOU</name>
<name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name>
<name sortKey="Wei Zhou" sort="Wei Zhou" uniqKey="Wei Zhou" last="Wei Zhou">WEI ZHOU</name>
<name sortKey="Ying Wan" sort="Ying Wan" uniqKey="Ying Wan" last="Ying Wan">YING WAN</name>
<name sortKey="Yuzhang Wu" sort="Yuzhang Wu" uniqKey="Yuzhang Wu" last="Yuzhang Wu">YUZHANG WU</name>
<name sortKey="Zhengcai Jia" sort="Zhengcai Jia" uniqKey="Zhengcai Jia" last="Zhengcai Jia">ZHENGCAI JIA</name>
</country>
</tree>
</affiliations>
</record>

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Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS

Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS

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